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Plasmid Ti

Peta plasmid Ti.

Plasmid Ti adalah vektor alamiah yang digunakan untuk mentransfer DNA ke dalam sel tanaman.[1] Bakteri yang membawa plasmid Ti (contohnya: Agrobacterium tumefaciens) dapat menyebabkan tumor pada tanaman yang disebut crown gall, terutama tanaman dikotil.[1][2] Pada sebagian besar plasmid Ti, terdapat lima kompleks gen, yaitu T-DNA (bagian yang ditransfer dan menyatu dengan genom tanaman sehingga), gen virulen (vir) yang terdiri dari 50 kilo-basa untuk mengatur proses transfer T-DNA ke dalam DNA tanaman, gen tra/trb yang mengatur perpindahan plasmid Ti antarbakteri, bagian yang mengatur sistem replikasi plasmid, dan bagian gen yang menyandikan molekul opin.[2] Molekul opin ini akan dihasilkan oleh jaringan tanaman yang terinfeksi bakteri pembawa plasmid Ti.[1]

Referensi

  1. ^ a b c (Inggris) P. Zambryskit, H. Joost, C. Genetellol, J. Leemans, M. Van Montagu, J. Schell (1983). "Ti plasmid vector for the introduction of DNA into plant cells without alteration of their normal regeneration capacity". The EMBO Joumal (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC555426/pdf/emboj00265-0051.pdf). 2 (12): 2143–2150. 
  2. ^ a b (Inggris) Gregory Phillips, Barbara E. Funnell (2004). Plasmid biology. ASM Press. ISBN 978-1-55581-265-2. Page.455-458

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