Sap barat
Sap barat (disebut juga sap imun protein) adalah sebuah metode untuk mendeteksi protein pada sampel jaringan. Sap imun menggunakan elektroforesis gel untuk memisahkan protein asli atau perubahan oleh jarak polipeptida atau oleh struktur 3-D protein. Protein tersebut dikirim ke membran, di mana mereka dideteksi menggunakan antibodi untuk menargetkan protein.
Referensi
- Renart J, Reiser J, Stark GR. "Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera: a method for studying antibody specificity and antigen structure", Proc Natl Acad Sci U S A, 1979 Jul;76(7):3116-20. PMID: 91164 abstract Diarsipkan 2008-03-23 di Wayback Machine.
- Towbin H, Staehelin T, Gordon J. "Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications" Proc Natl Acad Sci U S A, 1979 Sep;76(9):4350-4. PMID: 388439 abstract Diarsipkan 2008-03-17 di Wayback Machine.
- Citation's Classic: Burnette
Pranala luar
- Western blot analysis of sub-cellular fractionated samples using the Odyssey Infrared Imaging System (a protocol) Diarsipkan 2010-04-20 di Wayback Machine.
- Western blot protocol including example buffers and reprobing procedure Diarsipkan 2007-11-14 di Wayback Machine.
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