2-deoxy-scyllo-inosose synthase
Enzyme
2-deoxy-scyllo -Inosose synthase (EC 4.2.3.124, btrC (gene) , neoC (gene) , kanC (gene) ) is an enzyme with systematic name D -glucose-6-phosphate phosphate-lyase (2-deoxy-scyllo -inosose-forming) .[ 1] [ 2] [ 3] [ 4] [ 5] [ 6] [ 7] This enzyme catalyses the following chemical reaction
D -glucose 6-phosphate
⇌ ⇌ -->
{\displaystyle \rightleftharpoons }
2-deoxy-L -scyllo -inosose + phosphate
This enzyme requires Co2+ .
References
^ Kudo F, Yamauchi N, Suzuki R, Kakinuma K (May 1997). "Kinetic isotope effect and reaction mechanism of 2-deoxy-scyllo-inosose synthase derived from butirosin-producing Bacillus circulans " . The Journal of Antibiotics . 50 (5): 424–8. doi :10.7164/antibiotics.50.424 . PMID 9207913 .
^ Kudo F, Hosomi Y, Tamegai H, Kakinuma K (February 1999). "Purification and characterization of 2-deoxy-scyllo-inosose synthase derived from Bacillus circulans . A crucial carbocyclization enzyme in the biosynthesis of 2-deoxystreptamine-containing aminoglycoside antibiotics" . The Journal of Antibiotics . 52 (2): 81–8. doi :10.7164/antibiotics.52.81 . PMID 10344560 .
^ Kudo F, Tamegai H, Fujiwara T, Tagami U, Hirayama K, Kakinuma K (June 1999). "Molecular cloning of the gene for the key carbocycle-forming enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics and its comparison with dehydroquinate synthase" . The Journal of Antibiotics . 52 (6): 559–71. doi :10.7164/antibiotics.52.559 . PMID 10470681 .
^ Huang Z, Kakinuma K, Eguchi T (April 2005). "Stereospecificity of hydride transfer in NAD+ -catalyzed 2-deoxy-scyllo -inosose synthase, the key enzyme in the biosynthesis of 2-deoxystreptamine-containing aminocyclitol antibiotics". Bioorganic Chemistry . 33 (2): 82–9. doi :10.1016/j.bioorg.2004.09.002 . PMID 15788164 .
^ Thuy ML, Kharel MK, Lamichhane R, Lee HC, Suh JW, Liou K, Sohng JK (April 2005). "Expression of 2-deoxy-scyllo-inosose synthase (kanA) from kanamycin gene cluster in Streptomyces lividans ". Biotechnology Letters . 27 (7): 465–70. doi :10.1007/s10529-005-2222-y . PMID 15928851 .
^ Tamegai H, Nango E, Koike-Takeshita A, Kudo F, Kakinuma K (July 2002). "Significance of the 20-kDa subunit of heterodimeric 2-deoxy-scyllo -inosose synthase for the biosynthesis of butirosin antibiotics in Bacillus circulans " . Bioscience, Biotechnology, and Biochemistry . 66 (7): 1538–45. doi :10.1271/bbb.66.1538 . PMID 12224638 .
^ Tamegai H, Sawada H, Nango E, Aoki R, Hirakawa H, Iino T, Eguchi T (2010). "Roles of a 20 kDa protein associated with a carbocycle-forming enzyme involved in aminoglycoside biosynthesis in primary and secondary metabolism" . Bioscience, Biotechnology, and Biochemistry . 74 (6): 1215–9. doi :10.1271/bbb.90961 . PMID 20530911 .
External links
Activity Regulation Classification Kinetics Types