The 2000 National Football League was the inaugural season of the country's first football league held on a national scale. The league was also known as the Nitol-Tata National Football League due to sponsorship reasons.
The league included the top three finishing clubs from the 1999 season of the country's top-tier football league at the time, the Dhaka Premier Division League (Dhaka League). The clubs were the Dhaka League champions Mohammedan SC, runners-up Muktijoddha Sangsad KC and third-place Abahani Limited Dhaka. Additionally, the district league champions of Chittagong (Chittagong Abahani), Khulna (Khulna Abahani KC), and Rajshahi (Rajshahi Abahani KC) also entered the league. Chaturanga Jubo Sangha from Sylhet won a play-off against the champions of Barisal to secure their place in the league. Netrokona-based Mohammedan SC was also granted permission to enter the league.[1]