The 2008–09 Czech First League, known as the Gambrinus liga for sponsorship reasons, was the sixteenth season of Czech Republic's top-tier of football. It began on 2 August 2008 and ended on 30 May 2009. Slavia Prague were the defending champions. Slavia secured their 17th title overall after a 3–1 win away against Viktoria Žižkov in 28th round. They were the first club to defend Czech title since the 2000–01 season.
^1 Zlin manager Josef Mazura was relieved of his duties after the 7th round of matches; Ladislav Minář took over as caretaker manager until the appointment of Stanislav Levý before the 12th round.[10]
^2 Brno appointed Aleš Křeček to the position of caretaker manager after the 11th round of matches until Miroslav Beránek took over in the winter break.[10]