The Eerste Divisie 2009–10 was the 54th season of the Eerste Divisie since its establishment in 1956.
The previous year's winners were VVV-Venlo; they, with runner-up RKC Waalwijk, after a promotion/relegation playoff win over De Graafschap, were promoted to the Eredivisie. Twenty teams took part: eighteen from the 2008–2009 season and relegated teams FC Volendam and De Graafschap. The season's champion was promoted to the Eredivisie, while eight other teams, the second- through fifth-place finishers and period winners, faced the Eredivisie's sixteenth- and seventeenth-place finishers in promotion/relegation playoffs. For the first time since 1971, Eerste Divisie clubs also faced relegation; the last- and second-to-last-place teams were relegated to the new Topklasse, in which the best teams from Dutch amateur football play. The 2009–10 Eerste Divisie was sponsored by the Belgian beer brand Jupiler and so the official name of the league was Jupiler League.
The league started with twenty teams, but was reduced to nineteen in January 2010 following the disbandment of debt-ridden HFC Haarlem. All league games involving HFC Haarlem were cancelled and the number of teams that was relegated was consequently reduced to one.
^Since De Graafschap has won the Eerste Divisie Title, there will not be any team from the 4th Period participating in the Promotion/relegation play-offs for Eredivisie.
Playoffs
Please note that the following teams: Sparta Rotterdam & Willem II joined the Eerste Divisie-teams for the playoffs, after finishing 16th and 17th in the Eredivisie.