Note: Number of caps and players' ages are indicated as of 2 February 2024 – the tournament's opening day. For players added to a squad during the tournament, their caps and age are indicated as of the date of their call-up.
On 26 January 2024, Nick Isiekwe withdrew from England's pre-tournament training camp, because of illness. He was replaced in the squad by Charlie Ewels.[3]
On 14 February 2024, George Martin and Manu Tuilagi were added to the England squad during the tournament's first fallow week, after recovering from injury.[4]
On 18 February 2024, Alex Mitchell withdrew from the squad, ahead of England's third round fixture with Scotland, because of a knee injury. He was replaced by Harry Randall.[5]
On 10 March 2024, Chandler Cunningham-South withdrew from the squad, ahead of England's fifth round fixture with France, because of a calf injury. He was replaced by Guy Pepper.[6]
On 4 February 2024, Dany Priso was added to the squad, after Reda Wardi was ruled out of the rest of the tournament with a fractured wrist, which was suffered during the opening round match against Ireland.[11]
On 5 March 2024, Marco Manfredi withdrew from the squad, ahead of Italy's fourth round fixture with Scotland, because of a calf injury. He was replaced by Luca Bigi.[19]