Draft:Russell Higuchi
Submission declined on 6 March 2026 by ChrysGalley (talk).
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This draft is not adequately supported by reliable sources. Wikipedia's verifiability policy requires that all content be supported by reliable sources.
Declined by Rambley 6 months ago.
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Comment: The sources are not backing the text and show signs of LLM suggestive thinking. So for example source 6 makes 2 passing references to Higuchi (carefully noting he was one of many) and does not use the word pioneering. Source 5 is an 800 pages book and yet no page number is provided. ChrysGalley (talk) 23:11, 6 March 2026 (UTC)
- Thank you for the detailed review and for pointing out the issues.
- I understand the concerns regarding the wording and the alignment between the text and the cited sources. I will revise the draft to ensure that all statements closely follow the wording and scope of the sources. In particular, I will remove evaluative terms that are not explicitly used in the references and will add page numbers for book sources such as *A-Z of Quantitative PCR*.
- Thank you again for the helpful feedback. I will update the draft accordingly. De La Rue Thomas (talk) 03:30, 7 March 2026 (UTC)
Russell G. Higuchi is a molecular biologist whose work contributed to the development of real-time polymerase chain reaction (real-time PCR).[1] In 2019, the Association for Molecular Pathology presented him with its Award for Excellence in Molecular Diagnostics.[2]
In 1992, Higuchi and his colleagues reported a method that allowed PCR amplification to be detected without opening the reaction tube. They added ethidium bromide to the reaction mixture and monitored the increase in fluorescence as double-stranded DNA accumulated.[3]
The following year, Higuchi and his colleagues described a quantitative assay that used a video camera to monitor several PCRs during thermocycling. The fluorescence curves reflected the accumulation of double-stranded DNA and were related to the amount of DNA present at the start of the reaction.[4]
A 2006 review credited Higuchi and his coauthors with developing real-time PCR in 1992.[1] In a 2013 retrospective on the history of PCR, Mikael Kubista also described how Higuchi's group used changes in fluorescence to follow amplification during the reaction.[5]
References
- ^ a b Kubista M, Andrade JM, Bengtsson M, et al. (2006). "The real-time polymerase chain reaction". Molecular Aspects of Medicine. 27 (2–3): 95–125. doi:10.1016/j.mam.2005.12.007. PMID 16460794.
- ^ "Association for Molecular Pathology Announces 2019 Award Recipients". Association for Molecular Pathology. 19 September 2019. Retrieved 28 July 2026 – via Newswise.
- ^ Higuchi, Russell; Dollinger, Gavin; Walsh, P. Sean; Griffith, Robert (1992). "Simultaneous amplification and detection of specific DNA sequences". Bio/Technology. 10 (4): 413–417. doi:10.1038/nbt0492-413. PMID 1368485.
- ^ Higuchi, Russell; Fockler, Carita; Dollinger, Gavin; Watson, Robert (1993). "Kinetic PCR analysis: Real-time monitoring of DNA amplification reactions". Bio/Technology. 11 (9): 1026–1030. doi:10.1038/nbt0993-1026.
- ^ Kubista, Mikael (1 November 2013). "PCR @ 30: 30 Years Young and Still Evolving". Genetic Engineering & Biotechnology News. Retrieved 28 July 2026.
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