Fh8

Fh8 is a small protease produced by the liver fluke Fasciola hepatica. This parasite infects human and animals, and may lead to death. This protease is a very small one, 8 kDa, as is produced and secreted by the parasite during the infection process through liver. This protein has been tested and used as a fusion tag to improve the solubilization and expression of recombinant proteins, due to its biochemical characteristics.

References

Fraga, H; Faria, TQ; Pinto, F; Almeida, A; Brito, RM; Damas, AM (Dec 2010). "FH8—a small EF-hand protein from Fasciola hepatica". FEBS J. 277 (24): 5072–85. doi:10.1111/j.1742-4658.2010.07912.x. PMID 21078120.
Silva, E; Castro, A; Lopes, A; Rodrigues, A; Dias, C; Conceição, A; Alonso, J; Correia; da Costa, JM; Bastos, M; Parra, F; Moradas-Ferreira, P; Silva, M (Aug 2004). "A recombinant antigen recognized by Fasciola hepatica-infected hosts". J Parasitol. 90 (4): 746–51. doi:10.1645/ge-136r. PMID 15357063.

Hydrolase: proteases (EC 3.4)

3.4.11-19: Exopeptidase

3.4.11	Aminopeptidase Alanine Arginyl Aspartyl Cystinyl Leucyl Glutamyl Methionyl 1 2 O
3.4.13	Dipeptidase 1 2 3
3.4.14	Dipeptidyl peptidase Cathepsin C Dipeptidyl peptidase-4 Tripeptidyl peptidase Tripeptidyl peptidase I Tripeptidyl peptidase II
3.4.15	Angiotensin-converting enzyme
3.4.16	Serine type carboxypeptidases: Cathepsin A DD-Transpeptidase
3.4.17	Metalloexopeptidases Carboxypeptidase A A1 A2 B C E Glutamate II
Other/ungrouped	Metalloexopeptidase

3.4.21-25: Endopeptidase

Other/ungrouped: Amyloid-beta precursor protein secretase

3.4.99: Unknown

Staphylokinase

v t e Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)

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