N,N-dimethylformamidase

In enzymology, a N,N-dimethylformamidase (EC 3.5.1.56) is an enzyme that catalyzes the chemical reaction

N,N-dimethylformamide + H₂O ${\displaystyle \rightleftharpoons }$ dimethylamine + formate

Thus, the two substrates of this enzyme are N,N-dimethylformamide and H₂O, whereas its two products are dimethylamine and formate.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is N,N-dimethylformamide amidohydrolase. Other names in common use include dimethylformamidase, and DMFase. This enzyme participates in glyoxylate and dicarboxylate metabolism. It employs one cofactor, iron.

• Schar HP, Holzmann W, Ramos Tombo GM, Ghisalba O (1986). "Purification and characterization of N,N-dimethylformamidase from Pseudomonas DMF 3/3". Eur. J. Biochem. 158 (3): 469–75. doi:10.1111/j.1432-1033.1986.tb09778.x. PMID 3732281.