Refractometry

Refractometry is the analytical method of measuring substances' refractive index (one of their fundamental physical properties) in order to, for instance, assess their composition or purity. A refractometer is the instrument used to measure refractive index ("RI"). Although refractometers are best known for measuring liquids, they are also used to measure gases and solids, such as glass and gemstones.

The RI of a substance is strongly influenced by temperature and the wavelength of light used to measure it; therefore, care must be taken to control or compensate for temperature differences and wavelength. RI measurements are usually reported at a reference temperature of 20 degrees Celsius, which is equal to 68 degrees Fahrenheit and is considered to be room temperature. A reference wavelength of 589.3 nm (the sodium D line) is most often used. Though RI is a dimensionless quantity, it is typically reported as nD20 (or n20
D ), where the "n" represents refractive index, the "D" denotes the wavelength, and the 20 denotes the reference temperature. Therefore, the refractive index of water at 20 degrees Celsius, taken at the Sodium D Line, would be reported as 1.3330 nD20.

Refractometers are frequently used by grape growers and kiwifruit growers for Brix testing of sucrose levels in their fruit. Refractometry is also used in the gelatin industry. To convert the RI of a gelatin sol (reported in Brix) to a gelatin concentration, one need only multiply by eight-tenths (0.8). A sol with a 10.0 RI would therefore be 8% gelatin by weight. This is known to be a reliable conversion for gelatin sols as low as 1% up to over 50%.

Types of refractometers

There are four main types of fluid refractometers: traditional handheld refractometers, digital handheld refractometers, Abbe refractometers, and inline process refractometers.

Further reading

Colin E. Webb, Julian D. C. Jones (2004). Handbook of Laser Technology and Applications. Taylor & Francis. pp. 1738–1740. ISBN 0-7503-0966-0.

Content Disclaimer

Informasi ini disarikan dari Wikipedia dan disajikan kembali untuk tujuan edukasi. Konten tersedia di bawah lisensi CC BY-SA 3.0. Kami tidak bertanggung jawab atas ketidakakuratan data yang bersumber dari kontribusi publik tersebut.

  1. The information displayed on this website is sourced in part or in whole from Wikipedia and has been adapted for the purpose of restating it. We strive to provide accurate and relevant information, however:
  2. There is no guarantee of absolute accuracy. Wikipedia is an open, collaborative project that can be edited by anyone, so information is subject to change.
  3. It is not intended to constitute professional advice. The content displayed is for informational and educational purposes only. For important decisions (e.g., medical, legal, or financial), please consult a professional.
  4. Content copyright. Wikipedia is licensed under the Creative Commons Attribution-ShareAlike License (CC BY-SA). This means that content may be reused with appropriate attribution and shared under a similar license.
  5. Responsible use. Any risk arising from the use of information from this website is entirely the responsibility of the user.