中介體與一些週期蛋白依賴性激酶結合 酵母菌的中介體，可見其中MED14 中介體 （Mediator）舊稱DRIP 或TRAP [ 1] [ 2] 真核生物 細胞核 中啟動基因轉錄 所需的蛋白質複合體 [ 3] [ 4] 罗杰·科恩伯格 發現[ 5] [ 6] RNA聚合酶Ⅱ 的C端 結構域 和轉錄因子 結合[ 7] 強化子 和啟動子 之間，組成轉錄前起始複合體 。RNA轉錄起始後中介體和轉錄因子不會馬上自啟動子脫離，而會維持在該處以繼續結合新的RNA聚合酶Ⅱ，開啟新一輪的轉錄[ 3]
中介體的組成蛋白因物種而異[ 3] 釀酒酵母 的中介體有21個核心蛋白，哺乳類的中介體則有約30個，許多中介體的蛋白具有內在無序結構域 [ 8] 週期蛋白依賴性激酶 （CDK）結合，形成轉錄前起始複合體時與中介體結合的CDK會自其脫落，使RNA聚合酶Ⅱ得以與中介體結合[ 9] 非編碼RNA ncRNA-a結合，與其功能有關[ 10]
除了調控mRNA （RNA聚合酶Ⅱ）的轉錄外，還有研究顯示酵母菌的中介體參與RNA聚合酶III 轉錄tRNA 的過程[ 11] [ 12] 中節 與端粒 的結構[ 3] MED15 MED23 MED25 訊息傳遞路徑 ，可能為各種訊息傳遞路徑最終藉由影響中介體的蛋白以調控基因表現[ 3] [ 13] [ 14]
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