Cresol Red
Names
Preferred IUPAC name
3,3-Bis(4-hydroxy-3-methylphenyl)-2,1λ6 -benzoxathiole-1,1(3H )-dione
Identifiers
ChEBI
ChemSpider
ECHA InfoCard
100.015.513
UNII
InChI=1S/C21H18O5S/c1-13-11-15(7-9-18(13)22)21(16-8-10-19(23)14(2)12-16)17-5-3-4-6-20(17)27(24,25)26-21/h3-12,22-23H,1-2H3
Y Key: OBRMNDMBJQTZHV-UHFFFAOYSA-N
Y InChI=1/C21H18O5S/c1-13-11-15(7-9-18(13)22)21(16-8-10-19(23)14(2)12-16)17-5-3-4-6-20(17)27(24,25)26-21/h3-12,22-23H,1-2H3
Key: OBRMNDMBJQTZHV-UHFFFAOYAD
Cc1cc(ccc1O)/C(=C/2C=CC(=O)C(=C2)C)c3ccccc3OS(O)=O
Properties
C21 H17 NaO5 S
Molar mass
382.43 g/mol
Except where otherwise noted, data are given for materials in their
standard state (at 25 °C [77 °F], 100 kPa).
Chemical compound
Cresol red (full name: o -cresolsulfonephthalein)[ 1] is a triarylmethane dye frequently used for monitoring the pH in aquaria .
Cresol red (pH indicator )
below pH 7.2
above pH 8.8
7.2
⇌
8.8
Molecular biology
Cresol red can be used in many common molecular biology reactions in place of other loading dyes. Cresol Red does not inhibit Taq polymerase to the same degree as other common loading dyes.
Color marker
Cresol red can also be used as an electrophoretic color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis . In a 1% agarose gel, it runs approximately at the size of a 125 base pair (bp) DNA molecule (it depends on the concentration of buffer and other component). Bromophenol blue and xylene cyanol can also be used for this purpose.
References
^ PubChem. "Cresol red" . pubchem.ncbi.nlm.nih.gov . Retrieved 2023-01-25 .
External links