miR-30 microRNA precursor is a small non-coding RNA that regulates gene expression. Animal microRNAs are transcribed as pri-miRNA (primary miRNA) of varying length which in turns are processed in the nucleus by Drosha into ~70 nucleotidestem-loop precursor called pre-miRNA (precursor miRNA) and subsequently processed by the Dicer enzyme to give a mature ~22 nucleotide product. In this case the mature sequence comes from both the 3' (miR-30)[1] and 5' (mir-97-6)[2] arms of the precursor. The products are thought to have regulatory roles through complementarity to mRNA.[3]
A screen of 17 miRNAs that have been predicted to regulate a number of breast cancer associated genes found variations in the microRNAs
miR-17 and miR-30c-1, these patients were noncarriers of BRCA1 or BRCA2 mutations, lending the possibility that familial breast cancer may be caused by variation in these miRNAs.[4]
Members of the miR-30 family have been found to be highly expressed in heart cells.[5]
Targets of miR-30
It has been shown that the integrin ITGB3 and the ubiquitin conjugating E2 enzyme UBC9 are downregulated by miR-30.[6] It has also been suggested that the TP53 protein may be a target of miR-30c and miR-30e. An immunoblot analysis revealed that p53 expression levels were elevated upon knockdown of miR-30c and miR-30e.[7]
^Duisters RF, Tijsen AJ, Schroen B, et al. (January 2009). "miR-133 and miR-30 regulate connective tissue growth factor: implications for a role of microRNAs in myocardial matrix remodeling". Circ. Res. 104 (2): 170–8, 6p following 178. doi:10.1161/CIRCRESAHA.108.182535. PMID19096030.
^Yu F, Deng H, Yao H, Liu Q, Su F, Song E (July 2010). "Mir-30 reduction maintains self-renewal and inhibits apoptosis in breast tumor-initiating cells". Oncogene. 29 (29): 4194–204. doi:10.1038/onc.2010.167. PMID20498642.
