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Spent Mushroom Substrate (SMS) is a composted growing medium that results from the mushroom growing process. The utilization of SMS for biofertilizer or soil conditioner would be an important point in green agriculture. The study revealed the lignocellulolytic activity from 20 isolates of Fungi and 13 isolates of Bacteria which were isolated from sawdust as a spent mushroom substrate of Pleurotus ostreatus. The selected microorganism then would be used as inocullant for the production of biofertilizer using SMS as a main material. The lignocellulolytic system consist of laccase, cellulase and xylanase activity was analyzed. The results shown that among 20 isolates of Fungi, the highest activity of laccase, cellulase, and xylanase was obtained from the isolates 2F1, 2F4 and 2F5. There was (6.153 U, 4.662 U, 3.791U) for laccase, (6.740, 3.711 U, 3.605 U) for cellulase and (6.870 U, 4.673 U, 3.773 U) for xylanase respectively for 2F1, 2F4 and 2F5. Furhtermore, the characterization of the highest lignocellulytic fungi was also conducted. The isolate 2F1 opimally grow in pH 5 at 40oC, isolate 2F4 in pH 9 at 30oC and isolate 2F5 in pH 5 at 30oC. The identification of isolated fungi are in the progress.

Journal Name BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
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Location Kota adm. jakarta selatan, Dki jakarta INDONESIA
Website berita_biologi| http://e-journal.biologi.lipi.go.id/index.php/berita_biologi|
ISSN ISSN : -, EISSN : -, DOI : -,
Core Subject Science, Agriculture,
Meta Subject Agriculture, Biological Sciences & Forestry, Biochemistry, Genetics & Molecular Biology,
Meta Desc
PenulisSetiarto, R Haryo Bimo , Saskiawan, Iwan
Subtitle Article BERITA BIOLOGI Vol 12, No 1 (2013)
Publisher ArticleResearch Center for Biology-Indonesian Institute of Sciences
View Articlehttps://e-journal.biologi.lipi…
Scholar Googlehttp://scholar.google.com/scholar?q=%2Bintitle%3A&…
DOIhttps://doi.org/10.14203/beritabiol…
DOI Number DOI: 10.14203/beritabiologi.v12i1.514
Download Article [1] https://e-journal.biologi.lipi.go.id/ind…
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